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Western blot(SDS PAGE) analysis of extracts from HACAT .Using Cytokeratin 13Rabbit mAb [AE97] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KRT13,K1C13_HUMAN,Keratin, type I cytoskeletal 13
UniProtID :P13646
MASS(da) :49,588
MW(kDa) :51 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cytokeratin 13
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HACAT
Tissue specificity :Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.