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Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using VimentinRabbit mAb [BK14] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :VIM,VIME_HUMAN,Vimentin
UniProtID :P08670
MASS(da) :53,652
MW(kDa) :53 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Vimentin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.., Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2..
Tissue specificity :Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines..
Subcellular locationi :Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Cell membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.