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alpha Actinin/ACTN1 Rabbit mAb [114G]Cat NO.: A73131

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using alpha Actinin/ACTN1Rabbit mAb [114G] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ACTN1,ACTN1_HUMAN,Alpha-actinin-1

UniProtID :P12814

MASS(da) :103,058

MW(kDa) :103 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human alpha Actinin/ACTN1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Subcellular locationi :Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line. Cell membrane. Cell junction. Cell projection, ruffle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.