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Western blot(SDS PAGE) analysis of extracts from HAP1 cells .Using ENT1Rabbit mAb [A2HT] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SLC29A1,ENT1,S29A1_HUMAN,Equilibrative nucleoside transporter 1
UniProtID :Q99808
MASS(da) :50,219
MW(kDa) :50 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ENT1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HAP1 cells
Function : Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
Tissue specificity :Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen..
Subcellular locationi :Basolateral cell membrane,Multi-pass membrane protein. Apical cell membrane,Multi-pass membrane protein. Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.