* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from HEK-293T cells .Using Lactate Dehydrogenase B/LDH-BRabbit mAb [3MQD] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LDHB,LDHB_HUMAN,L-lactate dehydrogenase B chain
UniProtID :P07195
MASS(da) :36,638
MW(kDa) :37 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Lactate Dehydrogenase B/LDH-B
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293T cells
Function : Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+)..
Tissue specificity :Predominantly expressed in aerobic tissues such as cardiac muscle..
Subcellular locationi :Cytoplasm. Mitochondrion inner membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.