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Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using PKA R2/PKR2Rabbit mAb [8T1Z] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PRKAR2A,PKR2,PRKAR2,KAP2_HUMAN,cAMP-dependent protein kinase type II-alpha regulatory subunit
UniProtID :P13861
MASS(da) :45,518
MW(kDa) :50 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PKA R2/PKR2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Tissue specificity :Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Subcellular locationi :Cytoplasm. Cell membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.