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Western blot(SDS PAGE) analysis of extracts from MCF7 CELLS.Using PPP2R5DRabbit mAb [A1AX] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PPP2R5D,2A5D_HUMAN,Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
UniProtID :Q14738
MASS(da) :69,992
MW(kDa) :70 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PPP2R5D
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF7 CELLS
Function : The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Tissue specificity :Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.