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Western blot(SDS PAGE) analysis of extracts from HepG2 cells .Using TGN46Rabbit mAb [X562] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TGOLN2,TGN46,TGN51,TGON2_HUMAN,Trans-Golgi network integral membrane protein 2
UniProtID :O43493
MASS(da) :45,880
MW(kDa) :75-100 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TGN46
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : May be involved in regulating membrane traffic to and from trans-Golgi network.
Tissue specificity :Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.