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Western blot(SDS PAGE) analysis of extracts from Human small intestine.Using Trefoil Factor 3Rabbit mAb [RN95] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TFF3,ITF,TFI,TFF3_HUMAN,Trefoil factor 3
UniProtID :Q07654
MASS(da) :8,641
MW(kDa) :9 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Trefoil Factor 3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human small intestine
Function : Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen)..
Tissue specificity :Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Also expressed in the hypothalamus where it is detected in paraventricular, periventricular and supraoptic nuclei (at protein level)..
Subcellular locationi :Secreted, extracellular space, extracellular matrix. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.