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Western blot(SDS PAGE) analysis of extracts from HEK293T cells.Using ECH1Rabbit mAb [0A2Y] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ECH1,ECH1_HUMAN,Delta-Delta-dienoyl-CoA isomerase, mitochondrial
UniProtID :Q13011
MASS(da) :35,816
MW(kDa) :33 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ECH1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK293T cells
Function : Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA..
Subcellular locationi :Mitochondrion. Peroxisome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.