E-Cadherin Mouse mAb [5223]Cat NO.: A72774

Protein names ：CDH1,CDHE,UVO,CADH1_HUMAN,Cadherin-1 [Cleaved into: E-Cad/CTF1,E-Cad/CTF2,E-Cad/CTF3]

UniProtID ：P12830

MASS(da) ：97,456

MW(kDa) ：135 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：mouse

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human E-Cadherin

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells

Function ： Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells,cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.., E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.., (Microbial infection) Serves as a receptor for Listeria monocytogenes,internalin A (InlA) binds to this protein and promotes uptake of the bacteria..

Tissue specificity ：Non-neural epithelial tissues.

Subcellular locationi ：Cell junction, adherens junction. Cell membrane,Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.