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CD80 Rabbit mAb [15SU]Cat NO.: A31298

Western blot(SDS PAGE) analysis of extracts from Ramos cells .Using CD80Rabbit mAb [15SU] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CD80,CD28LG,CD28LG1,LAB7,CD80_HUMAN,T-lymphocyte activation antigen CD80

UniProtID :P33681

MASS(da) :33,048

MW(kDa) :60 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CD80

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Ramos cells 

Function : Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.., (Microbial infection) Acts as a receptor for adenovirus subgroup B..

Tissue specificity :Expressed on activated B-cells, macrophages and dendritic cells.

Subcellular locationi :Membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.