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Mitofilin Rabbit mAb [U04U]Cat NO.: A62272

Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using MitofilinRabbit mAb [U04U] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :IMMT,HMP,MIC60,MINOS2,PIG4,PIG52,MIC60_HUMAN,MICOS complex subunit MIC60

UniProtID :Q16891

MASS(da) :83,678

MW(kDa) :84 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Mitofilin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells

Function : Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:22114354, PubMed:25781180)..

Subcellular locationi :Mitochondrion inner membrane,Single-pass membrane protein. Mitochondrion.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.