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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using MitofilinRabbit mAb [U04U] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :IMMT,HMP,MIC60,MINOS2,PIG4,PIG52,MIC60_HUMAN,MICOS complex subunit MIC60
UniProtID :Q16891
MASS(da) :83,678
MW(kDa) :84 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Mitofilin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:22114354, PubMed:25781180)..
Subcellular locationi :Mitochondrion inner membrane,Single-pass membrane protein. Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.