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Western blot(SDS PAGE) analysis of extracts from Wild-type HAP1 cells.Using GST3 / GST piRabbit mAb [T79P] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GSTP1,FAEES3,GST3,GSTP1_HUMAN,Glutathione S-transferase P
UniProtID :P09211
MASS(da) :23,356
MW(kDa) :23 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GST3 / GST pi
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Wild-type HAP1 cells
Function : Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276). Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration..
Subcellular locationi :Cytoplasm. Mitochondrion. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.