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Western blot(SDS PAGE) analysis of extracts from Human fetal kidney.Using UFSP2Rabbit mAb [7R34] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :UFSP2,C4orf20,UFSP2_HUMAN,Ufm1-specific protease 2
UniProtID :Q9NUQ7
MASS(da) :53,261
MW(kDa) :53 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human UFSP2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human fetal kidney
Function : Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of UFM1, a ubiquitin-like modifier protein bound to a number of target proteins (PubMed:25219498, PubMed:32160526). Does not hydrolyze SUMO1 or ISG15 ubiquitin-like proteins (PubMed:25219498). Through TRIP4 deufmylation may regulate intracellular nuclear receptors transactivation and thereby regulate cell proliferation and differentiation (PubMed:25219498)..
Subcellular locationi :Cytoplasm. Endoplasmic reticulum. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.