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Western blot(SDS PAGE) analysis of extracts from Wild-type HAP1 cells.Using ACSS2Rabbit mAb [T1AH] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ACSS2,ACAS2,ACSA_HUMAN,Acetyl-coenzyme A synthetase, cytoplasmic
UniProtID :Q9NR19
MASS(da) :78,580
MW(kDa) :78 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ACSS2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Wild-type HAP1 cells
Function : Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed:10843999, PubMed:28003429). Acetate is the preferred substrate (PubMed:10843999, PubMed:28003429). Can also utilize propionate with a much lower affinity (By similarity)..
Subcellular locationi :Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.