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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using Peroxiredoxin 3/PRDX3Rabbit mAb [0Z41] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PRDX3,AOP1,PRDX3_HUMAN,Thioredoxin-dependent peroxide reductase, mitochondrial
UniProtID :P30048
MASS(da) :27,693
MW(kDa) :26 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Peroxiredoxin 3/PRDX3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed:7733872, PubMed:17707404). Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed:12492477)..
Subcellular locationi :Mitochondrion. Cytoplasm. Early endosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.