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Western blot(SDS PAGE) analysis of extracts from Jurkat cells .Using PlectinRabbit mAb [F0K5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PLEC,PLEC1,PLEC_HUMAN,Plectin
UniProtID :Q15149
MASS(da) :531,791
MW(kDa) :532 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Plectin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity..
Tissue specificity :Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
Subcellular locationi :Cytoplasm, cytoskeleton. Cell junction, hemidesmosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.