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Plectin Rabbit mAb [F0K5]Cat NO.: A43753

Western blot(SDS PAGE) analysis of extracts from Jurkat cells .Using PlectinRabbit mAb [F0K5] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PLEC,PLEC1,PLEC_HUMAN,Plectin

UniProtID :Q15149

MASS(da) :531,791

MW(kDa) :532  kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Plectin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Jurkat cells 

Function : Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity..

Tissue specificity :Widely expressed with highest levels in muscle, heart, placenta and spinal cord.

Subcellular locationi :Cytoplasm, cytoskeleton. Cell junction, hemidesmosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.