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SNAP29 Rabbit mAb [R375]Cat NO.: A48415

Western blot(SDS PAGE) analysis of extracts from Jurkat cells .Using SNAP29Rabbit mAb [R375] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SNAP29,SNP29_HUMAN,Synaptosomal-associated protein 29

UniProtID :O95721

MASS(da) :28,970

MW(kDa) :29 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human SNAP29

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Jurkat cells 

Function : SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Plays also a role in ciliogenesis by regulating membrane fusions..

Tissue specificity :Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas..

Subcellular locationi :Cytoplasm. Golgi apparatus membrane,Peripheral membrane protein. Cytoplasmic vesicle, autophagosome membrane,Peripheral membrane protein. Cell projection, cilium membrane,Peripheral membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.