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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using ADKRabbit mAb [AHK5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ADK,ADK_HUMAN,Adenosine kinase
UniProtID :P55263
MASS(da) :40,545
MW(kDa) :41 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ADK
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Catalyzes the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides..
Tissue specificity :Widely expressed. Highest level in placenta, liver, muscle and kidney..
Subcellular locationi :[Isoform 1]: Nucleus.,[Isoform 2]: Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.