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Western blot(SDS PAGE) analysis of extracts from Human fetal liver .Using CD73Rabbit mAb [ZWU4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NT5E,NT5,NTE,5NTD_HUMAN,5'-nucleotidase
UniProtID :P21589
MASS(da) :63,368
MW(kDa) :63 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CD73
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human fetal liver
Function : Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities..
Subcellular locationi :Cell membrane,Lipid-anchor, GPI-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.