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Western blot(SDS PAGE) analysis of extracts from MDA-MB-231 cells.Using MyoferlinRabbit mAb [7GJ1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MYOF,FER1L3,KIAA1207,MYOF_HUMAN,Myoferlin
UniProtID :Q9NZM1
MASS(da) :234,709
MW(kDa) :180,250 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Myoferlin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MDA-MB-231 cells
Function : Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR (By similarity)..
Tissue specificity :Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain..
Subcellular locationi :Cell membrane,Single-pass type II membrane protein. Nucleus membrane,Single-pass type II membrane protein. Cytoplasmic vesicle membrane,Single-pass type II membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.