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Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using CDKN2A/p14ARFRabbit mAb [YYC6] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDKN2A,CDKN2,MTS1,CDN2A_HUMAN,Cyclin-dependent kinase inhibitor 2A
UniProtID :P42771
MW(kDa) :14 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CDKN2A/p14ARF
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein..
Tissue specificity :Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific..
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.