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Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using Connexin 43 / GJA1Rabbit mAb [80O4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GJA1,GJAL,CXA1_HUMAN,Gap junction alpha-1 protein
UniProtID :P17302
MASS(da) :43,008
MW(kDa) :26-32 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Connexin 43 / GJA1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity)..
Tissue specificity :Expressed in the heart and fetal cochlea..
Subcellular locationi :Cell membrane,Multi-pass membrane protein. Cell junction, gap junction. Endoplasmic reticulum.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.