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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using ERp29Rabbit mAb [833K] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ERP29,C12orf8,ERP28,ERP29_HUMAN,Endoplasmic reticulum resident protein 29
UniProtID :P30040
MASS(da) :28,993
MW(kDa) :29 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ERp29
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.
Tissue specificity :Ubiquitous. Mostly expressed in secretory tissues.
Subcellular locationi :Endoplasmic reticulum lumen. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.