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Western blot(SDS PAGE) analysis of extracts from Wild-type HAP1 cells.Using EEF1A2Rabbit mAb [39OA] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EEF1A2,EEF1AL,STN,EF1A2_HUMAN,Elongation factor 1-alpha 2
UniProtID :Q05639
MASS(da) :50,470
MW(kDa) :50 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human EEF1A2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Wild-type HAP1 cells
Function : This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Tissue specificity :Brain, heart, and skeletal muscle.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.