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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using CMT2Rabbit mAb [V6VZ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MAD2L1BP,CMT2,KIAA0110,MD2BP_HUMAN,MAD2L1-binding protein )
UniProtID :Q15013
MASS(da) :31,052
MW(kDa) :34 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CMT2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex..
Subcellular locationi :Nucleus. Cytoplasm, cytoskeleton, spindle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.