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Collagen IV Rabbit mAb [8UJ6]Cat NO.: A32499

Western blot(SDS PAGE) analysis of extracts from Wild-type HeLa cells .Using Collagen IVRabbit mAb [8UJ6] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :COL4A1,CO4A1_HUMAN,Collagen alpha-1 chain [Cleaved into: Arresten]

UniProtID :P02462

MASS(da) :160,611

MW(kDa) :200 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Collagen IV

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Wild-type HeLa cells 

Function : Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.., Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation..

Tissue specificity :Highly expressed in placenta..

Subcellular locationi :Secreted, extracellular space, extracellular matrix, basement membrane.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.