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Western blot(SDS PAGE) analysis of extracts from Wild-type HeLa cells .Using Collagen IVRabbit mAb [8UJ6] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :COL4A1,CO4A1_HUMAN,Collagen alpha-1 chain [Cleaved into: Arresten]
UniProtID :P02462
MASS(da) :160,611
MW(kDa) :200 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Collagen IV
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Wild-type HeLa cells
Function : Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.., Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation..
Tissue specificity :Highly expressed in placenta..
Subcellular locationi :Secreted, extracellular space, extracellular matrix, basement membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.