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Western blot(SDS PAGE) analysis of extracts from MCF7 CELLS.Using Rab4Rabbit mAb [01M9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RAB4A,RAB4,RAB4A_HUMAN,Ras-related protein Rab-4A
UniProtID :P20338
MASS(da) :24,390
MW(kDa) :25 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Rab4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF7 CELLS
Function : Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state (PubMed:15907487, PubMed:16034420). Involved in protein transport (PubMed:29425100). Plays a role in vesicular traffic. Mediates VEGFR2 endosomal trafficking to enhance VEGFR2 signaling (PubMed:29425100). Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets (By similarity)..
Subcellular locationi :Membrane,Peripheral membrane protein. Cytoplasm. Early endosome membrane,Peripheral membrane protein. Recycling endosome membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.