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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using ERGIC3Rabbit mAb [341H] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ERGIC3,C20orf47,ERV46,SDBCAG84,CGI-54,PRO0989,ERGI3_HUMAN,Endoplasmic reticulum-Golgi intermediate compartment protein 3
UniProtID :Q9Y282
MASS(da) :43,222
MW(kDa) :50 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ERGIC3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Possible role in transport between endoplasmic reticulum and Golgi. Positively regulates trafficking of the secretory proteins SERPINA1/alpha1-antitrypsin and HP/haptoglobin (PubMed:31142615)..
Subcellular locationi :Endoplasmic reticulum-Golgi intermediate compartment membrane,Multi-pass membrane protein. Golgi apparatus, cis-Golgi network membrane,Multi-pass membrane protein. Endoplasmic reticulum membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.