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TGF beta 1 Rabbit mAb [Y9V7]Cat NO.: A86247

Western blot(SDS PAGE) analysis of extracts from L-929.Using TGF beta 1Rabbit mAb [Y9V7] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TGFB1,TGFB,TGFB1_HUMAN,Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ,Transforming growth factor beta-1 ]

UniProtID :P01137

MASS(da) :44,341

MW(kDa) :12.5,50 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TGF beta 1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :L-929

Tissue specificity :Highly expressed in bone (PubMed:11746498, PubMed:17827158). Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA) (PubMed:11746498, PubMed:17827158). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage (PubMed:17827158)..

Subcellular locationi :[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.,[Transforming growth factor beta-1]: Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.