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Western blot(SDS PAGE) analysis of extracts from L-929.Using TGF beta 1Rabbit mAb [Y9V7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TGFB1,TGFB,TGFB1_HUMAN,Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ,Transforming growth factor beta-1 ]
UniProtID :P01137
MASS(da) :44,341
MW(kDa) :12.5,50 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TGF beta 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :L-929
Tissue specificity :Highly expressed in bone (PubMed:11746498, PubMed:17827158). Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA) (PubMed:11746498, PubMed:17827158). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage (PubMed:17827158)..
Subcellular locationi :[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.,[Transforming growth factor beta-1]: Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.