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Peroxiredoxin 5 Rabbit mAb [YJF1]Cat NO.: A53810

Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using Peroxiredoxin 5Rabbit mAb [YJF1] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PRDX5,ACR1,SBBI10,PRDX5_HUMAN,Peroxiredoxin-5, mitochondrial

UniProtID :P30044

MASS(da) :22,086

MW(kDa) :17 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Peroxiredoxin 5

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells

Function : Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events..

Tissue specificity :Widely expressed..

Subcellular locationi :[Isoform Mitochondrial]: Mitochondrion.,[Isoform Cytoplasmic+peroxisomal]: Cytoplasm. Peroxisome matrix.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.