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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using Peroxiredoxin 5Rabbit mAb [YJF1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PRDX5,ACR1,SBBI10,PRDX5_HUMAN,Peroxiredoxin-5, mitochondrial
UniProtID :P30044
MASS(da) :22,086
MW(kDa) :17 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Peroxiredoxin 5
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events..
Tissue specificity :Widely expressed..
Subcellular locationi :[Isoform Mitochondrial]: Mitochondrion.,[Isoform Cytoplasmic+peroxisomal]: Cytoplasm. Peroxisome matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.