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Western blot(SDS PAGE) analysis of extracts from HuvEc cells.Using DDAH1Rabbit mAb [Z81P] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DDAH1,DDAH,DDAH1_HUMAN,N,N-dimethylarginine dimethylaminohydrolase 1
UniProtID :O94760
MASS(da) :31,122
MW(kDa) :37 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human DDAH1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HuvEc cells
Function : Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Tissue specificity :Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.