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Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using Ly-6KRabbit mAb [WLM9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LY6K,CO16,LY6K_HUMAN,Lymphocyte antigen 6K
UniProtID :Q17RY6
MASS(da) :18,673
MW(kDa) :24 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Ly-6K
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Required for sperm migration into the oviduct and male fertility by controlling binding of sperm to zona pellucida (By similarity). May play a role in cell growth (PubMed:18089789)..
Tissue specificity :Specifically expressed in testis (at protein level)..
Subcellular locationi :Secreted. Cytoplasm. Cell membrane,Lipid-anchor, GPI-anchor. Cytoplasmic vesicle, secretory vesicle, acrosome. Membrane raft.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.