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Histone H1.0 Rabbit mAb [0579]Cat NO.: A28159

Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using Histone H1.0Rabbit mAb [0579] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :H1-0,H1F0,H1FV,H10_HUMAN,Histone H1.0 ) [Cleaved into: Histone H1.0, N-terminally processed]

UniProtID :P07305

MASS(da) :20,863

MW(kDa) :30 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Histone H1.0

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells 

Function : Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.

Subcellular locationi :Nucleus. Chromosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.