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Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using Histone H1.0Rabbit mAb [0579] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :H1-0,H1F0,H1FV,H10_HUMAN,Histone H1.0 ) [Cleaved into: Histone H1.0, N-terminally processed]
UniProtID :P07305
MASS(da) :20,863
MW(kDa) :30 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Histone H1.0
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Subcellular locationi :Nucleus. Chromosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.