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Western blot(SDS PAGE) analysis of extracts from Human fetal heart .Using SLC25A12Rabbit mAb [D14T] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SLC25A12,ARALAR1,CMC1_HUMAN,Calcium-binding mitochondrial carrier protein Aralar1
UniProtID :O75746
MASS(da) :74,762
MW(kDa) :75 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human SLC25A12
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human fetal heart
Function : Mitochondrial and calcium-binding carrier that catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane (PubMed:11566871, PubMed:25410934). May have a function in the urea cycle (PubMed:11566871)..
Tissue specificity :Expressed predominantly in the heart and skeletal muscle, weakly in brain and kidney..
Subcellular locationi :Mitochondrion inner membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.