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Western blot(SDS PAGE) analysis of extracts from 293 cells.Using Carbonic Anhydrase 12/CA12Rabbit mAb [VPR7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CA12,CAH12_HUMAN,Carbonic anhydrase 12
UniProtID :O43570
MASS(da) :39,451
MW(kDa) :40 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Carbonic Anhydrase 12/CA12
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293 cells
Function : Reversible hydration of carbon dioxide..
Tissue specificity :Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium (PubMed:26911677)..
Subcellular locationi :Membrane,Single-pass type I membrane protein. Cell membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.