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CD20 Rabbit mAb [W1GB]Cat NO.: A15841

Western blot(SDS PAGE) analysis of extracts from Ramos cells .Using CD20Rabbit mAb [W1GB] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :MS4A1,CD20,CD20_HUMAN,B-lymphocyte antigen CD20

UniProtID :P11836

MASS(da) :33,077

MW(kDa) :33 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CD20

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Ramos cells 

Function : B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602)..

Tissue specificity :Expressed on B-cells.

Subcellular locationi :Cell membrane,Multi-pass membrane protein. Cell membrane,Lipid-anchor.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.