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Western blot(SDS PAGE) analysis of extracts from Jurkat cells treated 40nM TPA and 2uM calcium ionophore A23187 for 4 hours.Using NUR77Rabbit mAb [3SL7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NR4A1,GFRP1,HMR,NAK1,NR4A1_HUMAN,Nuclear receptor subfamily 4 group A member 1
UniProtID :P22736
MASS(da) :64,463
MW(kDa) :32,38,60-80 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human NUR77
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : Jurkat cells treated 40nM TPA and 2uM calcium ionophore A23187 for 4 hours
Function : Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Plays a role in the vascular response to injury (By similarity)..
Tissue specificity :Fetal muscle and adult liver, brain and thyroid.
Subcellular locationi :Nucleus. Cytoplasm. Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.