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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using MYL9Rabbit mAb [U9RA] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MYL9,MLC2,MRLC1,MYRL2,MYL9_HUMAN,Myosin regulatory light polypeptide 9
UniProtID :P24844
MASS(da) :19,827
MW(kDa) :14kD kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human MYL9
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (PubMed:11942626, PubMed:2526655). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity)..
Tissue specificity :Smooth muscle tissues and in some, but not all, nonmuscle cells..
Subcellular locationi :Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.