TNF alpha Rabbit mAb [438T]Cat NO.: A58672

Protein names ：TNF,TNFA,TNFSF2,TNFA_HUMAN,Tumor necrosis factor [Cleaved into: Tumor necrosis factor, membrane form ,Intracellular domain 1 ,Intracellular domain 2 ,C-domain 1,C-domain 2,Tumor necrosis factor, soluble form]

UniProtID ：P01375

MASS(da) ：25,644

MW(kDa) ：25 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TNF alpha

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：Raw 264.7 cells (treated with 100 ng/ml LPS for 6 hours with addition of 300 ng/ml BFA for the last 3 hours

Function ： Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819).., The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells..

Subcellular locationi ：Cell membrane,Single-pass type II membrane protein.,[Tumor necrosis factor, membrane form]: Membrane,Single-pass type II membrane protein.,[Tumor necrosis factor, soluble form]: Secreted.,[C-domain 1]: Secreted.,[C-domain 2]: Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.