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Western blot(SDS PAGE) analysis of extracts from HEK-293 cells.Using Alas1Rabbit mAb [XK8R] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ALAS1,ALAS3,ALASH,OK/SW-cl.121,HEM1_HUMAN,5-aminolevulinate synthase, non-specific, mitochondrial
UniProtID :P13196
MASS(da) :70,581
MW(kDa) :71 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Alas1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293 cells
Function : Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products..
Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.