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alpha Tubulin Rabbit mAb [GYRB]Cat NO.: A55548

Western blot(SDS PAGE) analysis of extracts from K562 cells .Using alpha TubulinRabbit mAb [GYRB] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TUBA1B,TBA1B_HUMAN,Tubulin alpha-1B chain [Cleaved into: Detyrosinated tubulin alpha-1B chain]

UniProtID :P68363

MASS(da) :50,152

MW(kDa) :50 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human alpha Tubulin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :K562 cells 

Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subcellular locationi :Cytoplasm, cytoskeleton.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.