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Western blot(SDS PAGE) analysis of extracts from K562 cells .Using alpha TubulinRabbit mAb [GYRB] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TUBA1B,TBA1B_HUMAN,Tubulin alpha-1B chain [Cleaved into: Detyrosinated tubulin alpha-1B chain]
UniProtID :P68363
MASS(da) :50,152
MW(kDa) :50 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human alpha Tubulin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cells
Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subcellular locationi :Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.