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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using TUGRabbit mAb [8G2P] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ASPSCR1,ASPL,RCC17,TUG,UBXD9,UBXN9,ASPC1_HUMAN,Tether containing UBX domain for GLUT4
UniProtID :Q9BZE9
MASS(da) :60,183
MW(kDa) :75 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TUG
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface (By similarity). Enhances VCP methylation catalyzed by VCPKMT..
Tissue specificity :Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas..
Subcellular locationi :Endomembrane system,Peripheral membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane,Peripheral membrane protein. Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.