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Western blot(SDS PAGE) analysis of extracts from human fetal liver.Using ACSL1Rabbit mAb [9646] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ACSL1,FACL1,FACL2,LACS,LACS1,LACS2,ACSL1_HUMAN,Long-chain-fatty-acid--CoA ligase 1
UniProtID :P33121
MASS(da) :77,943
MW(kDa) :78 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ACSL1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human fetal liver
Function : Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:24269233, PubMed:22633490, PubMed:21242590). Preferentially uses palmitoleate, oleate and linoleate (PubMed:24269233). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity)..
Tissue specificity :Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas..
Subcellular locationi :Mitochondrion outer membrane,Single-pass type III membrane protein. Peroxisome membrane,Single-pass type III membrane protein. Microsome membrane,Single-pass type III membrane protein. Endoplasmic reticulum membrane,Single-pass type III membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.