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beta Tubulin Rabbit mAb [EH20]Cat NO.: A62057

Western blot(SDS PAGE) analysis of extracts from Ramos cells.Using beta TubulinRabbit mAb [EH20] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TUBB,TUBB5,OK/SW-cl.56,TBB5_HUMAN,Tubulin beta chain

UniProtID :P07437

MASS(da) :49,671

MW(kDa) :55 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-5000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human beta Tubulin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Ramos cells

Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Tissue specificity :Ubiquitously expressed with highest levels in spleen, thymus and immature brain..

Subcellular locationi :Cytoplasm, cytoskeleton.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.