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Western blot(SDS PAGE) analysis of extracts from THP-1 cells .Using CD64Rabbit mAb [WQCU] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FCGR1A,FCG1,FCGR1,IGFR1,FCGR1_HUMAN,High affinity immunoglobulin gamma Fc receptor I
UniProtID :P12314
MASS(da) :42,632
MW(kDa) :43 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CD64
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :THP-1 cells
Function : High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells..
Tissue specificity :Monocyte/macrophage specific.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.