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Western blot(SDS PAGE) analysis of extracts from SK-MEL-2 cells8 cells.Using CD200 Rabbit mAb [YPRU] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CD200,MOX1,MOX2,My033,OX2G_HUMAN,OX-2 membrane glycoprotein
UniProtID :P41217
MASS(da) :31,264
MW(kDa) :45-50 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human CD200
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SK-MEL-2 cells8 cells
Function : Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.