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Western blot(SDS PAGE) analysis of extracts from HAP1 cells.Using NeurofibrominRabbit mAb [C970] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NF1,NF1_HUMAN,Neurofibromin [Cleaved into: Neurofibromin truncated]
UniProtID :P21359
MASS(da) :319,372
MW(kDa) :200,250,300 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Neurofibromin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HAP1 cells
Function : Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity..
Tissue specificity :Detected in brain, peripheral nerve, lung, colon and muscle..
Subcellular locationi :Nucleus. Nucleus, nucleolus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.