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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Aldehyde dehydrogenase 10Rabbit mAb [V39N] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ALDH3A2,ALDH10,FALDH,AL3A2_HUMAN,Aldehyde dehydrogenase family 3 member A2
UniProtID :P51648
MASS(da) :54,848
MW(kDa) :50 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Aldehyde dehydrogenase 10
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length (PubMed:9133646, PubMed:22633490, PubMed:25047030, PubMed:18035827, PubMed:9662422, PubMed:18182499). Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid (PubMed:22633490)..
Tissue specificity :Detected in liver (at protein level)..
Subcellular locationi :Microsome membrane,Single-pass membrane protein. Endoplasmic reticulum membrane,Single-pass membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.